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resource source identifier goat polyclonal anti-gfp novus biologicals cat
Resource Source Identifier Goat Polyclonal Anti Gfp Novus Biologicals Cat, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+antibody+novus+biologicals+cat/GFP+Antibody/pm41533514-237-2-9
Average 96 stars, based on 132 article reviews
Resource Source Identifier Goat Polyclonal Anti Gfp Novus Biologicals Cat, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+antibody+novus+biologicals+cat/GFP+Antibody/pm41533514-237-2-9
Average 96 stars, based on 132 article reviews
resource source identifier goat polyclonal anti-gfp novus biologicals cat - by Bioz Stars,
2026-09
96/100 stars
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Virus:Article Title: BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions Article Snippet: DOI: https://doi.org/10.7554/eLife.37122 16 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm MolProbity Chen et al. (2010) http://molprobity. biochem.duke.edu/ RRID:SCR_014226 Software, algorithm Phenix Adams et al. (2010) https://www.phenixonline.org/ RRID:SCR_014224 Software, algorithm PyMOL Schrodinger https://pymol.org/2/ RRID:SCR_000305 Other Opti-MEM I Reduced Serum Medium, no phenol red Thermo-Fisher Cat#11058021 Other ViewPlate, White 96-well plate, clear bottom for tissue culture PerkinElmer Cat#6005181 Other BRET2 Dual Emission optical module PerkinElmer Cat#2100–8140 Other Envision instrument, Multilabel Reader PerkinElmer Cat#2103 Article Title: A Targeted Protein Degradation Cell-Based Screening for Nanobodies Selective toward the Cellular RHOB GTP-Bound Conformation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies RHOA Mouse antibody Santa Cruz Biotechnology Cat#sc-418; RRID: AB_628218 RHOB Rabbit antibody Santa Cruz Biotechnology Cat#sc-180; RRID: AB_2179110 RHOC Rabbit antibody Cell Signaling Technology Cat#3430S; RRID: AB_2179246 Phospho-AKT S473 Rabbit antibody Cell Signaling Technology Cat#4058S; RRID: AB_331168 Total AKT1 Mouse antibody Cell Signaling Technology Cat#2967S; RRID: AB_331160 RAC1 Rabbit antibody Millipore Cat# 07-1464; RRID: AB_1977451 a-tubulin Mouse antibody Sigma-Aldrich Cat#T9026; RRID: AB_477593 CMYC HRP-linked Goat antibody Novus Biologicals Cat#NB600-341; RRID: AB_10000717 GAPDH (D4C6R) Mouse mAb Cell Signaling Technology Cat#97166; RRID: AB_2756824 g-H2AX Mouse antibody Cell Signaling Technology Cat#9718S; RRID: AB_2118009 Bacterial and Virus Strains E.coli BL21 (DE3) cells New England Biolabs Cat#C2527I E.coli XL1-Blue cells Stratagene Cat#200236 Chemicals, Peptides, and Recombinant Proteins RHOBQ63L This study N/A B6 VHH This study N/A GST-RBD This study N/A GST-PAKBD This study N/A Recombinant Human Epidermal Growth Factor (EGF) Sigma Cat#E9644 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8340 HaltTM Phosphatase Inhibitor Cocktail Thermo-Fisher Cat#1862495 Zeocin Invitrogen Cat#R25001 Bortezomib Selleckchem Cat#PS-341 MG-132 Sigma Cat#C2211 Critical Commercial Assays jetPRIME Polyplus Transfection Cat#114-15 CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat#G3582 Deposited Data RHOB-GDP structure Structural Genomics Consortium (SGC) PDB: 2FV8 RHOBQ63L-GTP structure This study PDB: 6HXU RHOBQ63L-GTP/B6 VHH structure This study PDB: 6SGE Experimental Models: Cell Lines Human: HEK293 cells ATCC Cat#CR-1573 Human: HeLa S3 cells ATCC Cat#CCL-2.2 Human: PC9 cells ECACC Cat#90071810 Human: NCI-H2882 cells Sato et al., 2007 RRID:CVCL_5158 Human: BEAS-2B cells ATCC Cat#CRL-9609 Recombinant DNA p-F-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-Ib-mGFP This study N/A p-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-H2B-mCherry-RHOBQ63LdeltaCAAX-IRES-Zeo This study N/A (Continued on next page) e1 Cell Chemical Biology 26, 1–15.e1–e6, November 21, 2019 Recombinant:Article Title: BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions Article Snippet: DOI: https://doi.org/10.7554/eLife.37122 16 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm MolProbity Chen et al. (2010) http://molprobity. biochem.duke.edu/ RRID:SCR_014226 Software, algorithm Phenix Adams et al. (2010) https://www.phenixonline.org/ RRID:SCR_014224 Software, algorithm PyMOL Schrodinger https://pymol.org/2/ RRID:SCR_000305 Other Opti-MEM I Reduced Serum Medium, no phenol red Thermo-Fisher Cat#11058021 Other ViewPlate, White 96-well plate, clear bottom for tissue culture PerkinElmer Cat#6005181 Other BRET2 Dual Emission optical module PerkinElmer Cat#2100–8140 Other Envision instrument, Multilabel Reader PerkinElmer Cat#2103 Article Title: A Targeted Protein Degradation Cell-Based Screening for Nanobodies Selective toward the Cellular RHOB GTP-Bound Conformation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies RHOA Mouse antibody Santa Cruz Biotechnology Cat#sc-418; RRID: AB_628218 RHOB Rabbit antibody Santa Cruz Biotechnology Cat#sc-180; RRID: AB_2179110 RHOC Rabbit antibody Cell Signaling Technology Cat#3430S; RRID: AB_2179246 Phospho-AKT S473 Rabbit antibody Cell Signaling Technology Cat#4058S; RRID: AB_331168 Total AKT1 Mouse antibody Cell Signaling Technology Cat#2967S; RRID: AB_331160 RAC1 Rabbit antibody Millipore Cat# 07-1464; RRID: AB_1977451 a-tubulin Mouse antibody Sigma-Aldrich Cat#T9026; RRID: AB_477593 CMYC HRP-linked Goat antibody Novus Biologicals Cat#NB600-341; RRID: AB_10000717 GAPDH (D4C6R) Mouse mAb Cell Signaling Technology Cat#97166; RRID: AB_2756824 g-H2AX Mouse antibody Cell Signaling Technology Cat#9718S; RRID: AB_2118009 Bacterial and Virus Strains E.coli BL21 (DE3) cells New England Biolabs Cat#C2527I E.coli XL1-Blue cells Stratagene Cat#200236 Chemicals, Peptides, and Recombinant Proteins RHOBQ63L This study N/A B6 VHH This study N/A GST-RBD This study N/A GST-PAKBD This study N/A Recombinant Human Epidermal Growth Factor (EGF) Sigma Cat#E9644 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8340 HaltTM Phosphatase Inhibitor Cocktail Thermo-Fisher Cat#1862495 Zeocin Invitrogen Cat#R25001 Bortezomib Selleckchem Cat#PS-341 MG-132 Sigma Cat#C2211 Critical Commercial Assays jetPRIME Polyplus Transfection Cat#114-15 CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat#G3582 Deposited Data RHOB-GDP structure Structural Genomics Consortium (SGC) PDB: 2FV8 RHOBQ63L-GTP structure This study PDB: 6HXU RHOBQ63L-GTP/B6 VHH structure This study PDB: 6SGE Experimental Models: Cell Lines Human: HEK293 cells ATCC Cat#CR-1573 Human: HeLa S3 cells ATCC Cat#CCL-2.2 Human: PC9 cells ECACC Cat#90071810 Human: NCI-H2882 cells Sato et al., 2007 RRID:CVCL_5158 Human: BEAS-2B cells ATCC Cat#CRL-9609 Recombinant DNA p-F-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-Ib-mGFP This study N/A p-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-H2B-mCherry-RHOBQ63LdeltaCAAX-IRES-Zeo This study N/A (Continued on next page) e1 Cell Chemical Biology 26, 1–15.e1–e6, November 21, 2019 Protease Inhibitor:Article Title: BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions Article Snippet: DOI: https://doi.org/10.7554/eLife.37122 16 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm MolProbity Chen et al. (2010) http://molprobity. biochem.duke.edu/ RRID:SCR_014226 Software, algorithm Phenix Adams et al. (2010) https://www.phenixonline.org/ RRID:SCR_014224 Software, algorithm PyMOL Schrodinger https://pymol.org/2/ RRID:SCR_000305 Other Opti-MEM I Reduced Serum Medium, no phenol red Thermo-Fisher Cat#11058021 Other ViewPlate, White 96-well plate, clear bottom for tissue culture PerkinElmer Cat#6005181 Other BRET2 Dual Emission optical module PerkinElmer Cat#2100–8140 Other Envision instrument, Multilabel Reader PerkinElmer Cat#2103 Article Title: A Targeted Protein Degradation Cell-Based Screening for Nanobodies Selective toward the Cellular RHOB GTP-Bound Conformation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies RHOA Mouse antibody Santa Cruz Biotechnology Cat#sc-418; RRID: AB_628218 RHOB Rabbit antibody Santa Cruz Biotechnology Cat#sc-180; RRID: AB_2179110 RHOC Rabbit antibody Cell Signaling Technology Cat#3430S; RRID: AB_2179246 Phospho-AKT S473 Rabbit antibody Cell Signaling Technology Cat#4058S; RRID: AB_331168 Total AKT1 Mouse antibody Cell Signaling Technology Cat#2967S; RRID: AB_331160 RAC1 Rabbit antibody Millipore Cat# 07-1464; RRID: AB_1977451 a-tubulin Mouse antibody Sigma-Aldrich Cat#T9026; RRID: AB_477593 CMYC HRP-linked Goat antibody Novus Biologicals Cat#NB600-341; RRID: AB_10000717 GAPDH (D4C6R) Mouse mAb Cell Signaling Technology Cat#97166; RRID: AB_2756824 g-H2AX Mouse antibody Cell Signaling Technology Cat#9718S; RRID: AB_2118009 Bacterial and Virus Strains E.coli BL21 (DE3) cells New England Biolabs Cat#C2527I E.coli XL1-Blue cells Stratagene Cat#200236 Chemicals, Peptides, and Recombinant Proteins RHOBQ63L This study N/A B6 VHH This study N/A GST-RBD This study N/A GST-PAKBD This study N/A Recombinant Human Epidermal Growth Factor (EGF) Sigma Cat#E9644 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8340 HaltTM Phosphatase Inhibitor Cocktail Thermo-Fisher Cat#1862495 Zeocin Invitrogen Cat#R25001 Bortezomib Selleckchem Cat#PS-341 MG-132 Sigma Cat#C2211 Critical Commercial Assays jetPRIME Polyplus Transfection Cat#114-15 CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat#G3582 Deposited Data RHOB-GDP structure Structural Genomics Consortium (SGC) PDB: 2FV8 RHOBQ63L-GTP structure This study PDB: 6HXU RHOBQ63L-GTP/B6 VHH structure This study PDB: 6SGE Experimental Models: Cell Lines Human: HEK293 cells ATCC Cat#CR-1573 Human: HeLa S3 cells ATCC Cat#CCL-2.2 Human: PC9 cells ECACC Cat#90071810 Human: NCI-H2882 cells Sato et al., 2007 RRID:CVCL_5158 Human: BEAS-2B cells ATCC Cat#CRL-9609 Recombinant DNA p-F-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-Ib-mGFP This study N/A p-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-H2B-mCherry-RHOBQ63LdeltaCAAX-IRES-Zeo This study N/A (Continued on next page) e1 Cell Chemical Biology 26, 1–15.e1–e6, November 21, 2019 Proliferation Assay:Article Title: BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions Article Snippet: DOI: https://doi.org/10.7554/eLife.37122 16 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm MolProbity Chen et al. (2010) http://molprobity. biochem.duke.edu/ RRID:SCR_014226 Software, algorithm Phenix Adams et al. (2010) https://www.phenixonline.org/ RRID:SCR_014224 Software, algorithm PyMOL Schrodinger https://pymol.org/2/ RRID:SCR_000305 Other Opti-MEM I Reduced Serum Medium, no phenol red Thermo-Fisher Cat#11058021 Other ViewPlate, White 96-well plate, clear bottom for tissue culture PerkinElmer Cat#6005181 Other BRET2 Dual Emission optical module PerkinElmer Cat#2100–8140 Other Envision instrument, Multilabel Reader PerkinElmer Cat#2103 Article Title: A Targeted Protein Degradation Cell-Based Screening for Nanobodies Selective toward the Cellular RHOB GTP-Bound Conformation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies RHOA Mouse antibody Santa Cruz Biotechnology Cat#sc-418; RRID: AB_628218 RHOB Rabbit antibody Santa Cruz Biotechnology Cat#sc-180; RRID: AB_2179110 RHOC Rabbit antibody Cell Signaling Technology Cat#3430S; RRID: AB_2179246 Phospho-AKT S473 Rabbit antibody Cell Signaling Technology Cat#4058S; RRID: AB_331168 Total AKT1 Mouse antibody Cell Signaling Technology Cat#2967S; RRID: AB_331160 RAC1 Rabbit antibody Millipore Cat# 07-1464; RRID: AB_1977451 a-tubulin Mouse antibody Sigma-Aldrich Cat#T9026; RRID: AB_477593 CMYC HRP-linked Goat antibody Novus Biologicals Cat#NB600-341; RRID: AB_10000717 GAPDH (D4C6R) Mouse mAb Cell Signaling Technology Cat#97166; RRID: AB_2756824 g-H2AX Mouse antibody Cell Signaling Technology Cat#9718S; RRID: AB_2118009 Bacterial and Virus Strains E.coli BL21 (DE3) cells New England Biolabs Cat#C2527I E.coli XL1-Blue cells Stratagene Cat#200236 Chemicals, Peptides, and Recombinant Proteins RHOBQ63L This study N/A B6 VHH This study N/A GST-RBD This study N/A GST-PAKBD This study N/A Recombinant Human Epidermal Growth Factor (EGF) Sigma Cat#E9644 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8340 HaltTM Phosphatase Inhibitor Cocktail Thermo-Fisher Cat#1862495 Zeocin Invitrogen Cat#R25001 Bortezomib Selleckchem Cat#PS-341 MG-132 Sigma Cat#C2211 Critical Commercial Assays jetPRIME Polyplus Transfection Cat#114-15 CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat#G3582 Deposited Data RHOB-GDP structure Structural Genomics Consortium (SGC) PDB: 2FV8 RHOBQ63L-GTP structure This study PDB: 6HXU RHOBQ63L-GTP/B6 VHH structure This study PDB: 6SGE Experimental Models: Cell Lines Human: HEK293 cells ATCC Cat#CR-1573 Human: HeLa S3 cells ATCC Cat#CCL-2.2 Human: PC9 cells ECACC Cat#90071810 Human: NCI-H2882 cells Sato et al., 2007 RRID:CVCL_5158 Human: BEAS-2B cells ATCC Cat#CRL-9609 Recombinant DNA p-F-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-Ib-mGFP This study N/A p-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-H2B-mCherry-RHOBQ63LdeltaCAAX-IRES-Zeo This study N/A (Continued on next page) e1 Cell Chemical Biology 26, 1–15.e1–e6, November 21, 2019 Plasmid Preparation:Article Title: BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions Article Snippet: DOI: https://doi.org/10.7554/eLife.37122 16 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm MolProbity Chen et al. (2010) http://molprobity. biochem.duke.edu/ RRID:SCR_014226 Software, algorithm Phenix Adams et al. (2010) https://www.phenixonline.org/ RRID:SCR_014224 Software, algorithm PyMOL Schrodinger https://pymol.org/2/ RRID:SCR_000305 Other Opti-MEM I Reduced Serum Medium, no phenol red Thermo-Fisher Cat#11058021 Other ViewPlate, White 96-well plate, clear bottom for tissue culture PerkinElmer Cat#6005181 Other BRET2 Dual Emission optical module PerkinElmer Cat#2100–8140 Other Envision instrument, Multilabel Reader PerkinElmer Cat#2103 Article Title: A Targeted Protein Degradation Cell-Based Screening for Nanobodies Selective toward the Cellular RHOB GTP-Bound Conformation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies RHOA Mouse antibody Santa Cruz Biotechnology Cat#sc-418; RRID: AB_628218 RHOB Rabbit antibody Santa Cruz Biotechnology Cat#sc-180; RRID: AB_2179110 RHOC Rabbit antibody Cell Signaling Technology Cat#3430S; RRID: AB_2179246 Phospho-AKT S473 Rabbit antibody Cell Signaling Technology Cat#4058S; RRID: AB_331168 Total AKT1 Mouse antibody Cell Signaling Technology Cat#2967S; RRID: AB_331160 RAC1 Rabbit antibody Millipore Cat# 07-1464; RRID: AB_1977451 a-tubulin Mouse antibody Sigma-Aldrich Cat#T9026; RRID: AB_477593 CMYC HRP-linked Goat antibody Novus Biologicals Cat#NB600-341; RRID: AB_10000717 GAPDH (D4C6R) Mouse mAb Cell Signaling Technology Cat#97166; RRID: AB_2756824 g-H2AX Mouse antibody Cell Signaling Technology Cat#9718S; RRID: AB_2118009 Bacterial and Virus Strains E.coli BL21 (DE3) cells New England Biolabs Cat#C2527I E.coli XL1-Blue cells Stratagene Cat#200236 Chemicals, Peptides, and Recombinant Proteins RHOBQ63L This study N/A B6 VHH This study N/A GST-RBD This study N/A GST-PAKBD This study N/A Recombinant Human Epidermal Growth Factor (EGF) Sigma Cat#E9644 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8340 HaltTM Phosphatase Inhibitor Cocktail Thermo-Fisher Cat#1862495 Zeocin Invitrogen Cat#R25001 Bortezomib Selleckchem Cat#PS-341 MG-132 Sigma Cat#C2211 Critical Commercial Assays jetPRIME Polyplus Transfection Cat#114-15 CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat#G3582 Deposited Data RHOB-GDP structure Structural Genomics Consortium (SGC) PDB: 2FV8 RHOBQ63L-GTP structure This study PDB: 6HXU RHOBQ63L-GTP/B6 VHH structure This study PDB: 6SGE Experimental Models: Cell Lines Human: HEK293 cells ATCC Cat#CR-1573 Human: HeLa S3 cells ATCC Cat#CCL-2.2 Human: PC9 cells ECACC Cat#90071810 Human: NCI-H2882 cells Sato et al., 2007 RRID:CVCL_5158 Human: BEAS-2B cells ATCC Cat#CRL-9609 Recombinant DNA p-F-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-Ib-mGFP This study N/A p-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-H2B-mCherry-RHOBQ63LdeltaCAAX-IRES-Zeo This study N/A (Continued on next page) e1 Cell Chemical Biology 26, 1–15.e1–e6, November 21, 2019 Software:Article Title: BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions Article Snippet: DOI: https://doi.org/10.7554/eLife.37122 16 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm MolProbity Chen et al. (2010) http://molprobity. biochem.duke.edu/ RRID:SCR_014226 Software, algorithm Phenix Adams et al. (2010) https://www.phenixonline.org/ RRID:SCR_014224 Software, algorithm PyMOL Schrodinger https://pymol.org/2/ RRID:SCR_000305 Other Opti-MEM I Reduced Serum Medium, no phenol red Thermo-Fisher Cat#11058021 Other ViewPlate, White 96-well plate, clear bottom for tissue culture PerkinElmer Cat#6005181 Other BRET2 Dual Emission optical module PerkinElmer Cat#2100–8140 Other Envision instrument, Multilabel Reader PerkinElmer Cat#2103 Article Title: A Targeted Protein Degradation Cell-Based Screening for Nanobodies Selective toward the Cellular RHOB GTP-Bound Conformation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies RHOA Mouse antibody Santa Cruz Biotechnology Cat#sc-418; RRID: AB_628218 RHOB Rabbit antibody Santa Cruz Biotechnology Cat#sc-180; RRID: AB_2179110 RHOC Rabbit antibody Cell Signaling Technology Cat#3430S; RRID: AB_2179246 Phospho-AKT S473 Rabbit antibody Cell Signaling Technology Cat#4058S; RRID: AB_331168 Total AKT1 Mouse antibody Cell Signaling Technology Cat#2967S; RRID: AB_331160 RAC1 Rabbit antibody Millipore Cat# 07-1464; RRID: AB_1977451 a-tubulin Mouse antibody Sigma-Aldrich Cat#T9026; RRID: AB_477593 CMYC HRP-linked Goat antibody Novus Biologicals Cat#NB600-341; RRID: AB_10000717 GAPDH (D4C6R) Mouse mAb Cell Signaling Technology Cat#97166; RRID: AB_2756824 g-H2AX Mouse antibody Cell Signaling Technology Cat#9718S; RRID: AB_2118009 Bacterial and Virus Strains E.coli BL21 (DE3) cells New England Biolabs Cat#C2527I E.coli XL1-Blue cells Stratagene Cat#200236 Chemicals, Peptides, and Recombinant Proteins RHOBQ63L This study N/A B6 VHH This study N/A GST-RBD This study N/A GST-PAKBD This study N/A Recombinant Human Epidermal Growth Factor (EGF) Sigma Cat#E9644 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8340 HaltTM Phosphatase Inhibitor Cocktail Thermo-Fisher Cat#1862495 Zeocin Invitrogen Cat#R25001 Bortezomib Selleckchem Cat#PS-341 MG-132 Sigma Cat#C2211 Critical Commercial Assays jetPRIME Polyplus Transfection Cat#114-15 CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat#G3582 Deposited Data RHOB-GDP structure Structural Genomics Consortium (SGC) PDB: 2FV8 RHOBQ63L-GTP structure This study PDB: 6HXU RHOBQ63L-GTP/B6 VHH structure This study PDB: 6SGE Experimental Models: Cell Lines Human: HEK293 cells ATCC Cat#CR-1573 Human: HeLa S3 cells ATCC Cat#CCL-2.2 Human: PC9 cells ECACC Cat#90071810 Human: NCI-H2882 cells Sato et al., 2007 RRID:CVCL_5158 Human: BEAS-2B cells ATCC Cat#CRL-9609 Recombinant DNA p-F-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-Ib-mGFP This study N/A p-Ib-6xHis-myc-IRES-Mito-mGFP This study N/A p-H2B-mCherry-RHOBQ63LdeltaCAAX-IRES-Zeo This study N/A (Continued on next page) e1 Cell Chemical Biology 26, 1–15.e1–e6, November 21, 2019 |